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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPA2B1 Recombinant monoclonal antibody (86713-1-MR)

    WB analysis using 86713-1-MR (same clone as 86713-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HNRNPA2B1 Recombinant monoclonal antibody (86713-1-MR)

    IHC staining of human colon using 86713-1-MR (same clone as 86713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HNRNPA2B1 Recombinant monoclonal antibody (86713-1-MR)

    IHC staining of human colon using 86713-1-MR (same clone as 86713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:15000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNRNPA2B1 Recombinant monoclonal antibody (86713-1-MR)

    IF Staining of HepG2 using 86713-1-MR (same clone as 86713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPA2B1 antibody (86713-1-MR, Clone: V3H6F7F2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPA2B1 Recombinant monoclonal antibody (86713-1-MR)

    IF Staining of HeLa using 86713-1-MR (same clone as 86713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA2B1 antibody (86713-1-MR, Clone: V3H6F7F2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86713-1-PBS in a different storage buffer formulation.

HNRNPA2B1 Recombinant monoclonal antibody, PBS Only

HNRNPA2B1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86713-1-PBS
Clone No.V3H6F7F2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPB1, HNRPA2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86713-1-PBS targets HNRNPA2B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6699

Product name: Recombinant human HNRNPA2B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-249 aa of BC000506

Sequence: MEREKEQFRKLFIGGLSFETTEESLRNYYEQWGKLTDCVVMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREESGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGKKRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKALSRQEMQEDLEVAILEVAPVMEEEEEDMVVEDLDMATRVGATEVVMTTMEEEIMEVEITMILEIITSNLLTTVQ

相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A2/B1
Calculated molecular weight 37 kDa
Observed molecular weight 34, 36 kDa
GenBank accession numberBC000506
Gene Symbol HNRNPA2B1
Gene ID (NCBI) 3181
RRIDAB_3745072
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP22626
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (36kd) and HNRNPB1 (38kd), by alternative splicing.

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