Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 11874-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11874-1-AP
WB result of HNMT antibody (11874-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNMT transfected HepG2 cells.
WB analysis of human liver using 11874-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11874-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 11874-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11874-1-AP (HNMT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11874-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11874-1-AP (HNMT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 11874-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 11874-1-AP (HNMT antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 11874-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HNMT antibody (11874-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

HNMT Polyclonal antibody

HNMT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11874-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HNMT S2, HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-29 cells, human liver tissue, mouse liver tissue, HepG2 cells
Positive IHC detected in	human liver cancer tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

11874-1-AP targets HNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2462 Product name: Recombinant human HNMT protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-292 aa of BC020677 Sequence: MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGDENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA 相同性解析による交差性が予測される生物種
Full Name	histamine N-methyltransferase
Calculated molecular weight	292 aa, 33 kDa
Observed molecular weight	30-33 kDa
GenBank accession number	BC020677
Gene Symbol	HNMT
Gene ID (NCBI)	3176
RRID	AB_2117048
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P50135
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.

Protocols

Product Specific Protocols
WB protocol for HNMT antibody 11874-1-AP	Download protocol
IHC protocol for HNMT antibody 11874-1-AP	Download protocol
IF protocol for HNMT antibody 11874-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Glia Molecular mechanism of histamine clearance by primary human astrocytes. Authors - Yoshikawa Takeo T View Article
human	WB	Cell Stem Cell Glioblastoma stem cell-specific histamine secretion drives pro-angiogenic tumor microenvironment remodeling Authors - Jiayi Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11874-1-AP

WB analysis of HepG2 using 11874-1-AP

WB analysis of human liver using 11874-1-AP

WB analysis of mouse liver using 11874-1-AP

IHC staining of human liver cancer using 11874-1-AP