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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HN1L Polyclonal antibody (16285-1-AP)

    WB analysis using 16285-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16285-1-AP (HN1L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using HN1L Polyclonal antibody (16285-1-AP)

    IHC staining of human stomach using 16285-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16285-1-AP (HN1L antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HN1L Polyclonal antibody (16285-1-AP)

    IF Staining of U2OS using 16285-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using HN1L antibody (16285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HN1L Polyclonal antibody (16285-1-AP)

    IF Staining of U2OS using 16285-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HN1L antibody (16285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HN1L Polyclonal antibody

HN1L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16285-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C16orf34, Hematological and neurological expressed 1-like protein, HN1-like protein, JPT2, Jupiter microtubule associated homolog 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16285-1-AP targets HN1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9400

Product name: Recombinant human HN1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-190 aa of BC014438

Sequence: MFQVPDSEGGRAGSRAMKPPGGESSNLFGSPEEATPSSRPNRMASNIFGPTEEPQNIPKRTNPPGGKGSGIFDESTPVQTRQHLNPPGGKTSDIFGSPVTATSRLAHPNKPKDHVFLCEGEEPKSDLKAARSIPAGAEPGEKGSARKAGPAKEQEPMPTVDSHEPRLGPRPRSHNKVLNPPGGKSSISFY

相同性解析による交差性が予測される生物種
Full Name hematological and neurological expressed 1-like
Calculated molecular weight 190 aa, 20 kDa
Observed molecular weight20-25 kDa
GenBank accession numberBC014438
Gene Symbol HN1L
Gene ID (NCBI) 90861
RRIDAB_3085484
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hematological and neurological expressed 1-like protein (HN1L), also known as Jupiter microtubule-associated homolog 2 (JPT2), belongs to the jupiter family. HN1L is nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed:33758061). HN1L enables NAADP to activate Ca2+ release from the endoplasmic reticulum through ryanodine receptors (PubMed:33758062). HN1L was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal systemHN1L is expressed in liver, kidney, prostate, testis and uterus (PMID: 15094197).

Protocols

Product Specific Protocols
IF protocol for HN1L antibody 16285-1-APDownload protocol
IHC protocol for HN1L antibody 16285-1-APDownload protocol
WB protocol for HN1L antibody 16285-1-APDownload protocol
Standard Protocols
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