Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 14914-1-AP (same clone as 14914-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
WB analysis of LNCaP using 14914-1-AP (same clone as 14914-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 14914-1-AP (same clone as 14914-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 14914-1-AP (same clone as 14914-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 14914-1-AP (same clone as 14914-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 14914-1-AP (same clone as 14914-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 14914-1-AP (same clone as 14914-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 14914-1-AP (same clone as 14914-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
IP experiment of MCF-7 using 14914-1-AP (same clone as 14914-1-PBS)
IP result of anti-HN1 (IP:14914-1-AP, 4ug; Detection:14914-1-AP 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 14914-1-AP (same clone as 14914-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 14914-1-AP (same clone as 14914-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

HN1 Polyclonal antibody, PBS Only

HN1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14914-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14914-1-PBS targets HN1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6712 Product name: Recombinant human HN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC001420 Sequence: MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPTEQPVRKNKMASNIFGTPEENQASWAKSAGAKSSGGREDLESSGLQRRNSSEASSGDFLDLKGEGDIHENVDTDLPGSLGQSEEKPVPAAPVPSPVAPAPVPSRRNPPGGKSSLVLG 相同性解析による交差性が予測される生物種
Full Name	hematological and neurological expressed 1
Calculated molecular weight	11 kDa, 16 kDa, 19 kDa
Observed molecular weight	23-30 kDa
GenBank accession number	BC001420
Gene Symbol	HN1
Gene ID (NCBI)	51155
RRID	AB_10863118
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UK76
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14914-1-AP (same clone as 14914-1-PBS)

WB analysis of LNCaP using 14914-1-AP (same clone as 14914-1-PBS)

WB analysis of mouse testis using 14914-1-AP (same clone as 14914-1-PBS)

WB analysis of mouse colon using 14914-1-AP (same clone as 14914-1-PBS)

WB analysis of human brain using 14914-1-AP (same clone as 14914-1-PBS)

WB analysis of human testis using 14914-1-AP (same clone as 14914-1-PBS)

WB analysis of HepG2 using 14914-1-AP (same clone as 14914-1-PBS)

WB analysis of A431 using 14914-1-AP (same clone as 14914-1-PBS)

IP experiment of MCF-7 using 14914-1-AP (same clone as 14914-1-PBS)

IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)