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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMHA1 Polyclonal antibody

HMHA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14832-1-AP

Synonyms

HA 1, HLA HA1, HMHA1, KIAA0223, mHag HA 1

Validation Data Gallery

Applications:
  • WB analysis using 14832-1-AP

    WB analysis using 14832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 14832-1-AP

    WB analysis of K-562 using 14832-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 14832-1-AP

    WB analysis of A375 using 14832-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 14832-1-AP

    WB analysis of mouse thymus using 14832-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsil using 14832-1-AP

    IHC staining of human tonsil using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human tonsil using 14832-1-AP

    IHC staining of human tonsil using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of mouse spleen using 14832-1-AP

    IHC staining of mouse spleen using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse spleen using 14832-1-AP

    IHC staining of mouse spleen using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human spleen using 14832-1-AP

    IHC staining of human spleen using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 14832-1-AP

    IHC staining of human spleen using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human spleen using 14832-1-AP

    IHC staining of human spleen using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 14832-1-AP

    IHC staining of human spleen using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse spleen using 14832-1-AP

    IHC staining of mouse spleen using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of mouse spleen using 14832-1-AP

    IHC staining of mouse spleen using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inA375 cells, K-562 cells, mouse thymus tissue, HeLa cells, Jurkat cells
Positive IHC detected inhuman tonsil tissue, human spleen tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

14832-1-AP targets HMHA1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMHA1 fusion protein Ag6636 相同性解析による交差性が予測される生物種
Full Name histocompatibility (minor) HA-1
Calculated molecular weight 1136 aa, 125 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC065223
Gene symbol HMHA1
Gene ID (NCBI) 23526
RRIDAB_10642145
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HMHA1 antibody 14832-1-APDownload protocol
IHC protocol for HMHA1 antibody 14832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Aging Dis

Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke

Authors - Hongyu Ma
View Article