Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24821-1-AP targets HMGXB4 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20424 Product name: Recombinant human HMGXB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-492 aa of BC104754 Sequence: SSSDAHSSPGPEGCGSDASQFAESHSANLDLSGLEPILVESDSSSGGELEAGELVIDDSYREIKKKKKSKKSKKKKDKEKHKEKRHSKSKRSLGLSAVPVGEVTVTSGPPPSIPYAGAAAPPLPLPGLHTDGHSEKKKKKEEKDKERERGEKPKKKNMSAYQVFCKEYRVTIVADHPGIDFGELSKKLAEVWKQLPEKDKLIWKQKAQYLQHKQNKAEATTVKRKASSSEGSMKVKASSVGVLSPQKKSPPTTMLLPASPAKAPETEPIDVAAHLQLLGESLSLIGHRLQETEGMVAVSGSLSVLLDSIICALGPLACLTTQLPELNGCPKQVLSNTLDNIAYIMPGL 相同性解析による交差性が予測される生物種 |
| Full Name | HMG box domain containing 4 |
| Calculated molecular weight | 601 aa, 66 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC104754 |
| Gene Symbol | HMGXB4 |
| Gene ID (NCBI) | 10042 |
| RRID | AB_2879742 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGU5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HMGXB4 also termed as HMG box containing protein 4 or HMG2L1 is a 601 amino acid protein, which contains one HMG box DNA-binding domain. HMGXB4 negatively regulates Wnt/beta-catenin signaling during development. HMG-2L1 is expressed in the nucleus and may play a role in transcriptional regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for HMGXB4 antibody 24821-1-AP | Download protocol |
| WB protocol for HMGXB4 antibody 24821-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
