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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HMGXB4 PolyClonal antibody (24821-1-AP)

    WB analysis of HeLa using 24821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24821-1-AP (HMGXB4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HMGXB4 PolyClonal antibody (24821-1-AP)

    WB analysis of Jurkat using 24821-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24821-1-AP (HMGXB4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HMGXB4 PolyClonal antibody (24821-1-AP)

    IP experiment of HeLa using 24821-1-AP

    IP result of anti-HMGXB4 (IP:24821-1-AP, 4ug; Detection:24821-1-AP 1:500) with HeLa cells lysate 2560ug.

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HMGXB4 Polyclonal antibody

HMGXB4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24821-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

HMG box containing protein 4, HMG box domain containing 4, HMG2L1, HMGBCG, HMGXB4, Protein HMGBCG, THC211630

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24821-1-AP targets HMGXB4 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGXB4 fusion protein Ag20424 相同性解析による交差性が予測される生物種
Full Name HMG box domain containing 4
Calculated molecular weight 601 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC104754
Gene Symbol HMGXB4
Gene ID (NCBI) 10042
RRIDAB_2879742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HMGXB4 also termed as HMG box containing protein 4 or HMG2L1 is a 601 amino acid protein, which contains one HMG box DNA-binding domain. HMGXB4 negatively regulates Wnt/beta-catenin signaling during development. HMG-2L1 is expressed in the nucleus and may play a role in transcriptional regulation.

Protocols

Product Specific Protocols
WB protocol for HMGXB4 antibody 24821-1-APDownload protocol
IP protocol for HMGXB4 antibody 24821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols