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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMGN2 Polyclonal antibody (10953-1-AP)

    WB analysis using 10953-1-AP (same clone as 10953-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HMGN2 Polyclonal antibody (10953-1-AP)

    IHC staining of human prostate cancer using 10953-1-AP (same clone as 10953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using HMGN2 Polyclonal antibody (10953-1-AP)

    IHC staining of human cervical cancer using 10953-1-AP (same clone as 10953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using HMGN2 Polyclonal antibody (10953-1-AP)

    IHC staining of human cervical cancer using 10953-1-AP (same clone as 10953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HMGN2 Polyclonal antibody (10953-1-AP)

    IHC staining of human prostate cancer using 10953-1-AP (same clone as 10953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HMGN2 Polyclonal antibody (10953-1-AP)

    IF Staining of HepG2 using 10953-1-AP (same clone as 10953-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGN2 antibody (10953-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

HMGN2 Polyclonal antibody, PBS Only

HMGN2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10953-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

High mobility group nucleosome-binding domain-containing protein 2, HMG17, Non-histone chromosomal protein HMG-17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10953-1-PBS targets HMGN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1401

Product name: Recombinant human HMGN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of BC014644

Sequence: MPKRKAEGDAKGDKAKVKDEPQRRSARLSAKPAPPKPEPKPKKAPAKKGEKVPKGKKGKADAGKEGNNPAENGDAKTDQAQKAEGAGDAK

相同性解析による交差性が予測される生物種
Full Name high-mobility group nucleosomal binding domain 2
Calculated molecular weight 9 aa, 3 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC014644
Gene Symbol HMGN2
Gene ID (NCBI) 3151
RRIDAB_2118482
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP05204
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.

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