Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 using 17643-1-AP
WB result of HMGCS1 antibody (17643-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMGCS1 transfected HepG2 cells.
WB analysis using 17643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 17643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 17643-1-AP
IP result of anti-HMGCS1 (IP:17643-1-AP, 4ug; Detection:17643-1-AP 1:3000) with HepG2 cells lysate 1480 ug.
IHC staining of human ovary cancer using 17643-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17643-1-AP (HMGCS1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17643-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMGCS1 antibody (17643-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

HMGCS1 Polyclonal antibody

HMGCS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17643-1-AP

Publications(47)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:2.3.3.10, HMG CoA synthase, HMG-CoA synthase, HMGCS, Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (47)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, A549 cells, HepG2 cells, LNCaP cells, mouse liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 44 publications below
IHC	See 7 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 3 publications below

Product Information

17643-1-AP targets HMGCS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11825 Product name: Recombinant human HMGCS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 171-520 aa of BC083514 Sequence: TGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTYHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH 相同性解析による交差性が予測される生物種
Full Name	3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
Calculated molecular weight	520 aa, 57 kDa
Observed molecular weight	57 kDa
GenBank accession number	BC083514
Gene Symbol	HMGCS1
Gene ID (NCBI)	3157
RRID	AB_2248359
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q01581
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).

Protocols

Product Specific Protocols
IF protocol for HMGCS1 antibody 17643-1-AP	Download protocol
IHC protocol for HMGCS1 antibody 17643-1-AP	Download protocol
IP protocol for HMGCS1 antibody 17643-1-AP	Download protocol
WB protocol for HMGCS1 antibody 17643-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis Authors - Wei Zhou KD Validated View Article
human	WB	Nat Cancer Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism Authors - Linjie Zhao KD Validated View Article
mouse	WB	Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Authors - Xiaohui Li View Article
mouse,human	WB,IF	J Cell Biol TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination. Authors - Wan Yun Ho View Article
mouse	WB	Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. Authors - Wei Wang View Article
human	WB	Mol Cell Proteomics Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression. Authors - Saraon Punit P View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 17643-1-AP

WB analysis using 17643-1-AP

WB analysis using 17643-1-AP

IP experiment of HepG2 using 17643-1-AP

IHC staining of human ovary cancer using 17643-1-AP

IF Staining of HepG2 using 17643-1-AP

HMGCS1 Polyclonal antibody

HMGCS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis

Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression.