Validation Data Gallery
Tested Applications
| Positive WB detected in | SKOV-3 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17206-1-AP targets HMGCLL1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10985 Product name: Recombinant human HMGCLL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC024194 Sequence: MGNVPSAVKHCLSYQQLLREHLWIGDSVAGALDPAQETSQLSGLPEFVKIVEVGPRDGLQNEKVIVPTDIKIEFINRLSQTGLSVIEVTSFVSSRWVPQMADHTEVMKGIHQYPGVRYPVLTPNLQGFHHAVAAGATEISVFGAASESFSKKNINCSIEESMGKFEEVVKSARHMNIPARGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIGVGTPGSMKRMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGLGLNTGVNLYKVMEAGDFICKAVNKTTNSKVAQASFNA 相同性解析による交差性が予測される生物種 |
| Full Name | 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1 |
| Calculated molecular weight | 340 aa, 36 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC024194 |
| Gene Symbol | HMGCLL1 |
| Gene ID (NCBI) | 54511 |
| RRID | AB_2878362 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TB92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HMGCLL1 (3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1), also named as er-cHL, is a close homolog of mitochondrial HMG-CoA lyase (mHL). HMGCLL1 catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, which is a key step in ketogenesis (PMID: 22847177, 22865860). HMGCLL1 has some isoforms with the molecular mass of 32-39 kDa and 6 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HMGCLL1 antibody 17206-1-AP | Download protocol |
| WB protocol for HMGCLL1 antibody 17206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
