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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMGB1 Recombinant antibody

HMGB1 Recombinant Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230182G6

Cat no : 82973-1-RR

Synonyms

DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

Validation Data Gallery

Applications:
  • WB analysis using 82973-1-RR

    WB analysis using 82973-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82973-1-RR (HMGB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 82973-1-RR

    IHC staining of mouse brain using 82973-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82973-1-RR (HMGB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMGB1 antibody included
  • IHC staining of mouse brain using 82973-1-RR

    IHC staining of mouse brain using 82973-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82973-1-RR (HMGB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMGB1 antibody included
  • IF Staining of HeLa using 82973-1-RR

    IF Staining of HeLa using 82973-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMGB1 antibody (82973-1-RR, Clone: 230182G6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). CoraLite®594-Phalloidin (red).

Tested Applications

Positive WB detected inL02 cells, Jurkat cells, HEK-293 cells, K-562 cells, HCT 116 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HMGB1 Ready-To-Use IHC Kit. HMGB1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:125-1:500
Sample-dependent, check data in validation data gallery

Product Information

82973-1-RR targets HMGB1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HMGB1 fusion protein Ag1264 相同性解析による交差性が予測される生物種
Full Name high-mobility group box 1
Calculated molecular weight 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC003378
Gene symbol HMGB1
Gene ID (NCBI) 3146
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HMGB1 antibody 82973-1-RRDownload protocol
IHC protocol for HMGB1 antibody 82973-1-RRDownload protocol
IF protocol for HMGB1 antibody 82973-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols