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Species Reactivity

Species Reactivity

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HMGA2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.781-12.5 ng/mL

Cat no : MP02971-1

Print datasheet

Synonyms

High mobility group AT-hook protein 2, High mobility group protein HMGI-C, HMGI C, HMGIC

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02971-1, Human HMGA2 Recombinant Matched Antibody Pair - PBS only. 87021-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14588. 87021-3-PBS was HRP conjugated as the detection antibody. Range: 0.781-12.5 ng/mL

    Sandwich ELISA standard curve of MP02971-1

    Sandwich ELISA standard curve of MP02971-1, Human HMGA2 Recombinant Matched Antibody Pair - PBS only. 87021-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14588. 87021-3-PBS was HRP conjugated as the detection antibody. Range: 0.781-12.5 ng/mL

  • The mean HMGA2 concentration was determined to be 335.16 ng/mL in SW480 cell extract based on a 3.0 mg/mL extract load, 859.24 ng/mL in HCT116 cell extract based on a 1.3 mg/mL extract load, 31.20 ng/mL in HT-29 cell extract based on a 1.1 mg/mL extract load and 88.90 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA sample test of MP02971-1

    The mean HMGA2 concentration was determined to be 335.16 ng/mL in SW480 cell extract based on a 3.0 mg/mL extract load, 859.24 ng/mL in HCT116 cell extract based on a 1.3 mg/mL extract load, 31.20 ng/mL in HT-29 cell extract based on a 1.1 mg/mL extract load and 88.90 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02971-1 targets HMGA2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.781-12.5 ng/mL (Sandwich ELISA);

Capture antibody: HMGA2 Recombinant monoclonal antibody, PBS Only (Capture) 87021-4-PBS (252110H2). 100 μg. Concentration 1 mg/ml.

Detection antibody: HMGA2 Recombinant monoclonal antibody, PBS Only (Detector) 87021-3-PBS (252110B4). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Ag14588

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 87021-4-PBS
Tested Reactivity human
Immunogen HMGA2 fusion protein Ag14588
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name high mobility group AT-hook 2
Synonyms
GenBank accession numberNM_003483
Gene symbol HMGA2
UniprotP52926
Gene ID (NCBI) 8091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 87021-3-PBS
Tested Reactivity human
Immunogen HMGA2 fusion protein Ag14588
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name high mobility group AT-hook 2
Synonyms
GenBank accession numberNM_003483
Gene symbol HMGA2
UniprotP52926
Gene ID (NCBI) 8091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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