• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMGA1 Polyclonal antibody

HMGA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15398-1-AP

Synonyms

high mobility group AT hook 1, High mobility group protein A1, High mobility group protein R, HMG I(Y), HMG R, HMGA1, HMGA1A, HMGIY

Validation Data Gallery

Applications:
  • WB analysis of COLO 320 using 15398-1-AP

    WB analysis of COLO 320 using 15398-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15398-1-AP (HMGA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15398-1-AP

    IHC staining of human prostate cancer using 15398-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15398-1-AP (HMGA1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15398-1-AP targets HMGA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGA1 fusion protein Ag7613 相同性解析による交差性が予測される生物種
Full Name high mobility group AT-hook 1
Calculated molecular weight 12 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC004924
Gene symbol HMGA1
Gene ID (NCBI) 3159
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Publications

SpeciesApplicationTitle
HamsterWB

Lipids

The Safety and Anti-Hypercholesterolemic Effect of Coptisine in Syrian Golden Hamsters.

Authors - Kai He
View Article