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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMG20B Monoclonal antibody (67354-1-Ig)

    WB analysis using 67354-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67354-1-Ig (HMG20B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HMG20B Monoclonal antibody (67354-1-Ig)

    IHC staining of human skin cancer using 67354-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HMG20B Monoclonal antibody (67354-1-Ig)

    IHC staining of human skin cancer using 67354-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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HMG20B Monoclonal antibody

HMG20B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67354-1-Ig
Clone No.4C2A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

4C2A9, BRAF25, BRAF35, BRCA2 associated factor 35, BRCA2-associated factor 35

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67354-1-Ig targets HMG20B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMG20B fusion protein Ag6103 相同性解析による交差性が予測される生物種
Full Name high-mobility group 20B
Calculated molecular weight 36 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC002552
Gene Symbol HMG20B
Gene ID (NCBI) 10362
RRIDAB_2882609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P0W2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation (PMID:10449787). The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse (PMID:15240883). HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure (PMID:19997604). The predicted molecular weight of the HMG20B protein is 36 kDa, while the molecular weight detected in the literature is approximately 40 kDa by SDS-PAGE (PMID: 36171271).

Protocols

Product Specific Protocols
WB protocol for HMG20B antibody 67354-1-IgDownload protocol
IHC protocol for HMG20B antibody 67354-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols