• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMBS Polyclonal antibody

HMBS Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14797-1-AP

Synonyms

HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 14797-1-AP

    WB analysis of HeLa using 14797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 14797-1-AP

    WB analysis of Raji using 14797-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 14797-1-AP

    WB analysis of HEK-293 using 14797-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 14797-1-AP

    WB analysis of A375 using 14797-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 14797-1-AP

    IP experiment of HEK-293 using 14797-1-AP

    IP result of anti-HMBS (IP:14797-1-AP, 4ug; Detection:14797-1-AP 1:700) with HEK-293 cells lysate 2400 ug.

  • IF Staining of MCF-7 using 14797-1-AP

    IF Staining of MCF-7 using 14797-1-AP

    Immunofluorescent analysis of MCF-7 cells, using HMBS antibody 14797-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of HeLa using 14797-1-AP

    IF Staining of HeLa using 14797-1-AP

    Immunofluorescent analysis of Hela cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of HepG2 using 14797-1-AP

    IF Staining of HepG2 using 14797-1-AP

    Immunofluorescent analysis of HepG2 cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, A375 cells, HEK-293 cells, Raji cells
Positive IP detected inHEK-293 cells
Positive IF detected inMCF-7 cells, Hela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14797-1-AP targets HMBS in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMBS fusion protein Ag6509 相同性解析による交差性が予測される生物種
Full Name hydroxymethylbilane synthase
Calculated molecular weight 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC000520
Gene symbol HMBS
Gene ID (NCBI) 3145
RRIDAB_2117447
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HMBS antibody 14797-1-APDownload protocol
IF protocol for HMBS antibody 14797-1-APDownload protocol
IP protocol for HMBS antibody 14797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols