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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    WB analysis using 17260-1-AP (same clone as 17260-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    WB analysis of MCF-7 using 17260-1-AP (same clone as 17260-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    WB analysis of HepG2 using 17260-1-AP (same clone as 17260-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    WB analysis of Jurkat using 17260-1-AP (same clone as 17260-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IP experiment of HepG2 using 17260-1-AP (same clone as 17260-1-PBS)

    IP result of anti-HLA class I (HLA-B) (IP:17260-1-AP, 4ug; Detection:17260-1-AP 1:4000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human stomach cancer using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human stomach cancer using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human spleen using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human spleen using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human stomach cancer using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human stomach cancer using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human tonsillitis using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human tonsillitis using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human heart using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human heart using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human kidney using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human kidney using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human lung using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human lung using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human ovary using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human ovary using 17260-1-AP (same clone as 17260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IF Staining of HepG2 using 17260-1-AP (same clone as 17260-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HLA class I (HLA-B) antibody (17260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

HLA class I (HLA-B) Polyclonal antibody, PBS Only

HLA class I (HLA-B) Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17260-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HLA-B, HLA B, HLA class I histocompatibility antigen, B alpha chain, Human leukocyte antigen B, MHC class I antigen

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17260-1-PBS targets HLA class I (HLA-B) in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10947

Product name: Recombinant human HLA class I (HLA-B) protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-362 aa of BC013187

Sequence: MRVTAPRTVLLLLSGALALTETWAGSHSMRYFYTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQICKTNTQTYRESLRNLRGYYNQSEAGSHTLQRMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTIPIVGIVAGLAVLAVVVIGAVVATVMCRRKSSGGKGGSYSQAASSDSAQGSDVSLTA

相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, B
Calculated molecular weight 362 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC013187
Gene Symbol HLA class I (HLA-B)
Gene ID (NCBI) 3106
RRIDAB_1939610
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP01889
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-B can also react with HLA-A and HLA-C. (PMID: 667938; 3375250)

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