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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DRB5 Polyclonal antibody

HLA-DRB5 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21702-1-AP

Synonyms

DR beta 2, DR beta 5, Dw2, HLA DRB, HLA DRB5, HLA-DRB5, MHC class II antigen DRB5

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 21702-1-AP

    WB analysis of HepG2 using 21702-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 21702-1-AP

    WB analysis of Raji using 21702-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 21702-1-AP

    WB analysis of HeLa using 21702-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 21702-1-AP

    WB analysis of HL-60 using 21702-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 21702-1-AP

    IHC staining of human tonsillitis using 21702-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 21702-1-AP

    IHC staining of human tonsillitis using 21702-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human tonsillitis using 21702-1-AP

    IHC staining of human tonsillitis using 21702-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 21702-1-AP

    IHC staining of human tonsillitis using 21702-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Raji using 21702-1-AP

    FC experiment of Raji using 21702-1-AP

    1X10^6 Raji cells were stained with 0.20ug HLA-DRB5 antibody (21702-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HL-60 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21702-1-AP is HLA-DRB5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRB5 fusion protein Ag16307 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR beta 5
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC009234
Gene symbol HLA-DRB5
Gene ID (NCBI) 3127
RRIDAB_2878905
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB5 antibody 21702-1-APDownload protocol
IHC protocol for HLA-DRB5 antibody 21702-1-APDownload protocol
FC protocol for HLA-DRB5 antibody 21702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols