Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16789-1-AP targets HLA-DRB3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10223 Product name: Recombinant human HLA-DRB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-228 aa of BC008987 Sequence: DTRPRFLELRKSECHFFNGTERVRYLDRYFHNQEEFLRFDSDVGEYRAVTELGRPVAESWNSQKDLLEQKRGRVDNYCRHNYGVGESFTVQRRVHPQVTVYPAKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSALTVEWRARSESAQSKM 相同性解析による交差性が予測される生物種 |
| Full Name | major histocompatibility complex, class II, DR beta 3 |
| Calculated molecular weight | 266 aa, 30 kDa |
| Observed molecular weight | 28-30 kDa |
| GenBank accession number | BC008987 |
| Gene Symbol | HLA-DRB3 |
| Gene ID (NCBI) | 3125 |
| RRID | AB_3085508 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P79483 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are heterodimers consisting of an alpha (DRA) and a beta (DRB) chain. HLA-DRB3 is a beta chain of MHC class II molecule expressed on the surface of antigen presenting cells (APC) like B cells, macrophages, and dendritic cells (PMID: 30192820). It is involved in adaptive immune responses by presenting peptide antigens to CD4+ T cells (PMID: 19830726).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HLA-DRB3 antibody 16789-1-AP | Download protocol |
| WB protocol for HLA-DRB3 antibody 16789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |