Validation Data Gallery
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Tested Applications
| Positive WB detected in | Raji cells, Daudi cells, Ramos cells, pig lung tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68543-1-Ig targets HLA-DRA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11224 Product name: Recombinant human HLA-DRA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 24-218 aa of BC032350 Sequence: WAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENV 相同性解析による交差性が予測される生物種 |
| Full Name | major histocompatibility complex, class II, DR alpha |
| Calculated molecular weight | 254 aa, 29 kDa |
| Observed molecular weight | 32-34 kDa |
| GenBank accession number | BC032350 |
| Gene Symbol | HLA-DRA |
| Gene ID (NCBI) | 3122 |
| ENSEMBL Gene ID | ENSG00000204287 |
| RRID | AB_3085250 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P01903 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HLA-DRA antibody 68543-1-Ig | Download protocol |
| IHC protocol for HLA-DRA antibody 68543-1-Ig | Download protocol |
| WB protocol for HLA-DRA antibody 68543-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |