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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using HLA-DQB2 Polyclonal antibody (17315-1-AP)

    WB analysis of Raji using 17315-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 17315-1-AP (HLA-DQB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HLA-DQB2 Polyclonal antibody (17315-1-AP)

    WB analysis of HepG2 using 17315-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17315-1-AP (HLA-DQB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using HLA-DQB2 Polyclonal antibody (17315-1-AP)

    WB analysis of K-562 using 17315-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17315-1-AP (HLA-DQB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HLA-DQB2 Polyclonal antibody

HLA-DQB2 Polyclonal Antibody for WB, ELISA
Cat No. 17315-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HLA DQB1, HLA DQB2, HLA DXB, HLA-DQB2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17315-1-AP targets HLA-DQB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11284

Product name: Recombinant human HLA-DQB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-231 aa of BC031995

Sequence: MSWKMALQIPGGFWAAAVTVMLVMLSTPVAEARDFPKDFLVQFKGMCYFTNGTERVRGVARYIYNREEYGRFDSDVGEFQAVTELGRSIEDWNNYKDFLEQERAAVDKVCRHNYEAELRTTLQRQVEPTVTISPSRTEALNHHNLLVCSVTDFYPAQIKVQWFRNDQEETAGVVSTSLIRNGDWTFQILVMLEITPQRGDIYTCQVEHPSLQSPITVEWRPRGPPPAGLLH

相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DQ beta 2
Calculated molecular weight231aa,27 kDa; 275aa,31 kDa
Observed molecular weight 27 kDa, 31 kDa
GenBank accession numberBC031995
Gene Symbol HLA-DQB2
Gene ID (NCBI) 3120
RRIDAB_1939611
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP05538
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DQB2 antibody 17315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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