Validation Data Gallery
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Tested Applications
| Positive WB detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
16918-1-AP targets HLA-DQA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10224 Product name: Recombinant human HLA-DQA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-220 aa of BC008585 Sequence: EDIVADHVASYGVNLYQSYGPSGQYTHEFDGDEQFYVDLGRKETVWCLPVLRQFRFDPQFALTNIAVLKHNLNSLIKRSNSTAATNEVPEVTVFSKSPVTLGQPNILICLVDNIFPPVVNITWLSNGHSVTEGVSETSFLSKSDHSFFKISYLTLLPSAEESYDCKVEHWGLDKPLLKHWEPEIPAPMSELTETVVC 相同性解析による交差性が予測される生物種 |
| Full Name | major histocompatibility complex, class II, DQ alpha 1 |
| Calculated molecular weight | 254 aa, 28 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC008585 |
| Gene Symbol | HLA-DQA1 |
| Gene ID (NCBI) | 3117 |
| RRID | AB_2878332 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01909 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HLA-DQA1 antibody 16918-1-AP | Download protocol |
| IHC protocol for HLA-DQA1 antibody 16918-1-AP | Download protocol |
| WB protocol for HLA-DQA1 antibody 16918-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Identification of proteomic signatures associated with COPD frequent exacerbators. | ||
iScience Single-cell transcriptomics reveals the role of antigen presentation in liver metastatic breast cancer | ||
Ital J Pediatr Amino acid variants in the HLA-DQA1 and HLA-DQB1 molecules explain the major association of variants with relapse status in pediatric patients with steroid-sensitive nephrotic syndrome | ||
Front Genet Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System. | ||
PLoS One Variations in the MHC region confer risk to esophageal squamous cell carcinoma on the subjects from high-incidence area in northern china. |