HLA-DMA Recombinant antibody
HLA-DMA Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
230155E7
Cat no : 82899-2-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Ramos cells, Raji cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Product Information
82899-2-RR targets HLA-DMA in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | HLA-DMA fusion protein Ag2452 相同性解析による交差性が予測される生物種 |
| Full Name | major histocompatibility complex, class II, DM alpha |
| Calculated molecular weight | 261 aa, 29 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC026279 |
| Gene symbol | HLA-DMA |
| Gene ID (NCBI) | 3108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HLA-DMA antibody 82899-2-RR | Download protocol |
| IHC protocol for HLA-DMA antibody 82899-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
