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Validation Data Gallery

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  • Western Blot (WB) analysis of rat spleen tissue using HLA-DMA Polyclonal antibody (11838-1-AP)

    WB analysis of rat spleen using 11838-1-AP (same clone as 11838-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 11838-1-AP (HLA-DMA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using HLA-DMA Polyclonal antibody (11838-1-AP)

    WB analysis of human liver using 11838-1-AP (same clone as 11838-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 11838-1-AP (HLA-DMA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DMA Polyclonal antibody (11838-1-AP)

    IHC staining of human tonsillitis using 11838-1-AP (same clone as 11838-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11838-1-AP (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DMA Polyclonal antibody (11838-1-AP)

    IHC staining of human tonsillitis using 11838-1-AP (same clone as 11838-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11838-1-AP (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

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HLA-DMA Polyclonal antibody, PBS Only

HLA-DMA Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11838-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

D6S222E, DMA, DMA protein, DMA protein MHC class II antigen Major histocompatibility complex, class II, DM alpha cDNA FLJ75740, highly similar to Homo sapiens major histocompatibility complex, class II, DM alpha, hCG_2001572

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11838-1-PBS targets HLA-DMA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DMA fusion protein Ag2452 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DM alpha
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC026279
Gene Symbol HLA-DMA
Gene ID (NCBI) 3108
RRIDAB_874186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28067
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides. This antibody recognizes the 33kd HLA-DMA and the 50-60kd dimer in Western blotting.