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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using HKR1 Polyclonal antibody (14902-1-AP)

    WB analysis of COLO 320 using 14902-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HEK-293 cells using HKR1 Polyclonal antibody (14902-1-AP)

    WB analysis of HEK-293 using 14902-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HKR1 Polyclonal antibody (14902-1-AP)

    WB analysis of HeLa using 14902-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using HKR1 Polyclonal antibody (14902-1-AP)

    WB analysis of COLO 320 using 14902-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

HKR1 Polyclonal antibody

HKR1 Polyclonal Antibody for WB, ELISA
Cat No. 14902-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GLI Kruppel family member HKR1, HKR1, Protein HKR1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14902-1-AP targets HKR1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6694

Product name: Recombinant human HKR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 254-599 aa of BC053845

Sequence: NLITHQRTHSGEKPYVCKDCGRGFTWKSNLFTHQRTHSGLKPYVCKECGQSFSLKSNLITHQRAHTGEKPYVCRECGRGFRQHSHLVRHKRTHSGEKPYICRECEQGFSQKSHLIRHLRTHTGEKPYVCTECGRHFSWKSNLKTHQRTHSGVKPYVCLECGQCFSLKSNLNKHQRSHTGEKPFVCTECGRGFTRKSTLSTHQRTHSGEKPFVCAECGRGFNDKSTLISHQRTHSGEKPFMCRECGRRFRQKPNLFRHKRAHSGAFVCRECGQGFCAKLTLIKHQRAHAGGKPHVCRECGQGFSRQSHLIRHQRTHSGEKPYICRKCGRGFSRKSNLIRHQRTHSG

相同性解析による交差性が予測される生物種
Full Name GLI-Kruppel family member HKR1
Calculated molecular weight 73 kDa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC053845
Gene Symbol HKR1
Gene ID (NCBI) 284459
RRIDAB_2878091
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10072
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HKR1, also named as ZNF875, belongs to the krueppel C2H2-type zinc-finger protein family. HRK1 is a modular protein that comprises a photosensory rhodopsin, a histidine kinase, a response regulator and a putative guanylyl cyclase domain. HKR1 is related to the protonation state of the Schiff base function that provides the linkage of the retinal chromophore with a Lys side chain of the protein (PMID: 25836735). HRK1 has 2 isoforms with the molecular mass of 73-75 kDa.

Protocols

Product Specific Protocols
WB protocol for HKR1 antibody 14902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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