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Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of human saliva using 67803-1-Ig (same clone as 67803-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of rat spleen using 67803-1-Ig (same clone as 67803-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of Raji using 67803-1-Ig (same clone as 67803-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of Ramos using 67803-1-Ig (same clone as 67803-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of RAW 264.7 using 67803-1-Ig (same clone as 67803-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HK3 Monoclonal antibody (67803-1-Ig)

    IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HK3 Monoclonal antibody (67803-1-Ig)

    IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

HK3 Monoclonal antibody, PBS Only

HK3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67803-1-PBS
Clone No.3H3C7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

EC:2.7.1.1, Hexokinase 3, hexokinase 3 (white cell), Hexokinase type III, Hexokinase-3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67803-1-PBS targets HK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30798

Product name: Recombinant human HK3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 622-923 aa of BC028129

Sequence: LLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV

相同性解析による交差性が予測される生物種
Full Name hexokinase 3 (white cell)
Calculated molecular weight 923 aa, 99 kDa
Observed molecular weight99 kDa
GenBank accession numberBC028129
Gene Symbol HK3
Gene ID (NCBI) 3101
RRIDAB_2918566
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP52790
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

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