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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Hexokinase 2 Recombinant antibody (85875-1-RR)

    WB analysis using 85875-1-RR (same clone as 85875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Hexokinase 2 Recombinant antibody (85875-1-RR)

    WB analysis using 85875-1-RR (same clone as 85875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02173-3

    Sandwich ELISA standard curve of MP02173-3

    Sandwich ELISA standard curve of MP02173-3, Human Hexokinase 2 Recombinant Matched Antibody Pair - PBS only. 85875-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16895. 85875-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP02173-3

    Sample test of MP02173-3

    The mean Hexokinase 2 concentration was determined to be 321.70 ng/mL in A549 cell extract based on a 1.3 mg/mL extract load.

  • Sample test of MP02173-3

    Sample test of MP02173-3

    Serum of sixteen individual healthy human donors was measured. The human Hexokinase 2 concentration of detected samples was determined to be 1.16 ng/mL with a range of 0.35 - 4.27 ng/mL.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Hexokinase 2 Recombinant antibody (85875-1-RR)

    IHC staining of mouse skeletal muscle using 85875-1-RR (same clone as 85875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Hexokinase 2 Recombinant antibody (85875-1-RR)

    IHC staining of mouse skeletal muscle using 85875-1-RR (same clone as 85875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85875-1-RR

    Affinity and Kinetic Characterization of 85875-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85875-1-RR against Human Hexokinase 2 were performed. The affinity constant is 0.302 nM.

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Hexokinase 2 Recombinant antibody, PBS Only (Detector)

Hexokinase 2 Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85875-1-PBS
Clone No.250190C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85875-1-PBS targets Hexokinase 2 as part of a matched antibody pair:

MP02173-1: 85875-1-PBS capture and 85875-2-PBS detection (validated in Cytometric bead array)

MP02173-2: 85875-1-PBS capture and 85875-3-PBS detection (validated in Cytometric bead array)

MP02173-3: 85875-3-PBS capture and 85875-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Hexokinase 2 fusion protein Ag16895 相同性解析による交差性が予測される生物種
Full Name hexokinase 2
Calculated molecular weight 102 kDa
Observed molecular weight 102-120 kDa
GenBank accession numberBC021116
Gene Symbol Hexokinase 2
Gene ID (NCBI) 3099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52789
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236).