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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HJURP Polyclonal antibody (15283-1-AP)

    WB analysis using 15283-1-AP (same clone as 15283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15283-1-AP (HJURP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using HJURP Polyclonal antibody (15283-1-AP)

    IHC staining of human brain using 15283-1-AP (same clone as 15283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using HJURP Polyclonal antibody (15283-1-AP)

    IHC staining of human brain using 15283-1-AP (same clone as 15283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15283-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HJURP Polyclonal antibody, PBS Only

HJURP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15283-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15283-1-PBS targets HJURP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HJURP fusion protein Ag7402 相同性解析による交差性が予測される生物種
Full Name Holliday junction recognition protein
Calculated molecular weight 86 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC001940
Gene Symbol HJURP
Gene ID (NCBI) 55355
RRIDAB_2116879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCD3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)