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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27740-1-AP

Synonyms

H2B clustered histone 26, H2B type 12, H2B3B, H2BC26, HIST3H2BB, histone cluster 3, H2bb, Histone H2B type 3 B

Validation Data Gallery

Applications:
  • WB analysis of HEK-293T using 27740-1-AP

    WB analysis of HEK-293T using 27740-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 27740-1-AP

    WB analysis using 27740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 27740-1-AP

    WB analysis of HEK-293 using 27740-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293T cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

27740-1-AP targets Histone H2B in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H2B fusion protein Ag26901 相同性解析による交差性が予測される生物種
Full Name histone cluster 3, H2bb
Observed molecular weight15-20 kDa
GenBank accession numberBC100855
Gene symbol HIST3H2BB
Gene ID (NCBI) 128312
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.

Protocols

Product Specific Protocols
WB protocol for Histone H2B antibody 27740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols