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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H2B Polyclonal antibody (27740-1-AP)

    WB analysis using 27740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using Histone H2B Polyclonal antibody (27740-1-AP)

    WB analysis of HEK-293T using 27740-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Histone H2B Polyclonal antibody (27740-1-AP)

    WB analysis of HEK-293 using 27740-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Histone H2B Polyclonal antibody (27740-1-AP)

    IF Staining of HEK-293 using 27740-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Histone H2B antibody (27740-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • ChIP experiment of HEK-293 cells using Histone H2B Polyclonal antibody (27740-1-AP)

    ChIP experiment of HEK-293 using 27740-1-AP

    Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 18 µg of cross-linked chromatin, 5 µg of Histone H2B Antibody (27740-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, IF/ICC, ChIP, ELISA
Cat No. 27740-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ChIP, ELISA

Synonyms

H2B3B, HIST3H2BB, H2B-clustered histone 26, H2BC26, H2B.U histone 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293T cells, HEK-293 cells
Positive IF/ICC detected inHEK-293 cells
Positive ChIP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Chromatin immunoprecipitation (ChIP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27740-1-AP targets Histone H2B in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26901

Product name: Recombinant human HIST3H2BB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-126 aa of BC100855

Sequence: MPDPSKSAPAPKKGSKKAVTKAQKKDGKKRKRGRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

相同性解析による交差性が予測される生物種
Full Name histone cluster 3, H2bb
Observed molecular weight15-20 kDa
GenBank accession numberBC100855
Gene Symbol Histone H2B
Gene ID (NCBI) 128312
RRIDAB_3085992
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N257
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.

Protocols

Product Specific Protocols
WB protocol for Histone H2B antibody 27740-1-APDownload protocol
IF protocol for Histone H2B antibody 27740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols