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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H1.5 Polyclonal antibody (18093-1-AP)

    WB analysis using 18093-1-AP (same clone as 18093-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (Histone H1.5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Histone H1.5 Polyclonal antibody (18093-1-AP)

    IHC staining of mouse colon using 18093-1-AP (same clone as 18093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Histone H1.5 Polyclonal antibody (18093-1-AP)

    IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Histone H1.5 Polyclonal antibody (18093-1-AP)

    IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Histone H1.5 Polyclonal antibody (18093-1-AP)

    IHC staining of mouse colon using 18093-1-AP (same clone as 18093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Histone H1.5 Polyclonal antibody (18093-1-AP)

    IHC staining of human colon using 18093-1-AP (same clone as 18093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Histone H1.5 Polyclonal antibody (18093-1-AP)

    IF Staining of SH-SY5Y using 18093-1-AP (same clone as 18093-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Histone H1.5 antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

Histone H1.5 Polyclonal antibody, PBS Only

Histone H1.5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18093-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18093-1-PBS targets Histone H1.5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12818

Product name: Recombinant human HIST1H1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-226 aa of BC101581

Sequence: MSETAPAETATPAPVEKSPAKKKATKKAAGAGAAKRKATGPPVSELITKAVAASKERNGLSLAALKKALAAGGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGATPKKAKKAAGAKKAVKKTPKKAKKPAAAGVKKVAKSPKKAKAAAKPKKATKSPAKPKAVKPKAAKPKAAKPKAAKPKAAKAKKAAAKKK

相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H1b
Calculated molecular weight 226 aa, 23 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC101581
Gene Symbol HIST1H1B
Gene ID (NCBI) 3009
RRIDAB_3085553
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP16401
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1.5, also known as Histone Cluster 1 H1 Family Member B (HIST1H1B), is a member of the Histone H1/H5 protein family. Histone H1.5 gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. Histone H1.5 expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).

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