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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIP14; ZDHHC17 Polyclonal antibody (30022-1-AP)

    WB analysis using 30022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HIP14; ZDHHC17 Polyclonal antibody (30022-1-AP)

    WB analysis using 30022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HIP14; ZDHHC17 Polyclonal antibody (30022-1-AP)

    IF Staining of U2OS using 30022-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HIP14; ZDHHC17 antibody (30022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

HIP14/ZDHHC17 Polyclonal antibody

HIP14/ZDHHC17 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30022-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HIP14; ZDHHC17, ZDHHC17, Acyltransferase ZDHHC17, DHHC 17, DHHC domain-containing cysteine-rich protein 17

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, Raji cells, U-251 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30022-1-AP targets HIP14/ZDHHC17 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32654

Product name: Recombinant human HIP14; ZDHHC17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-203 aa of BC050324

Sequence: MQREEGFNTKMADGPDEYDTEAGCVPLLHPEEIKPQSHYNHGYGEPLGRKTHIDDYSTWDIVKATQYGIYERCRELVEAGYDVRQPDKENVTLLHWAAINNRIDLVKYYISKGAIVDQLGGDLNSTPLHWATRQGHLSMVVQLMKYGADPSLIDGEGCSCIHLAAQFGHTSIVAYLIAKGQDVDMMDQNGMTPLMWAAYRTHS

相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 17
Calculated molecular weight 73 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC050324
Gene Symbol ZDHHC17
Gene ID (NCBI) 23390
RRIDAB_2935500
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUH5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZDHHC17 is a neuronal palmitoyl transferase. Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.

Protocols

Product Specific Protocols
WB protocol for HIP14/ZDHHC17 antibody 30022-1-APDownload protocol
IF protocol for HIP14/ZDHHC17 antibody 30022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols