Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of A431 using 27650-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 27650-1-AP
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 27650-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lung using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 27650-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 27650-1-AP (HIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 27650-1-AP
Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using HIF3A antibody (27650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

HIF3A Polyclonal antibody

HIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27650-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

bHLHe17, HIF 3 alpha, HIF 3A, HIF 3A2, HIF 3A4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Cobalt Chloride treated HeLa cells, A431 cells
Positive IHC detected in	human placenta tissue, mouse kidney tissue, human lung tissue, mouse cerebellum tissue, mouse lung tissue, rat cerebellum tissue, rat kidney tissue, rat lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Tunicamycin treated HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

27650-1-AP targets HIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26574 Product name: Recombinant human HIF3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 401-486 aa of NM_022462 Sequence: MALAADPRRFCSPDLRRLLGPILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDADALD 相同性解析による交差性が予測される生物種
Full Name	hypoxia inducible factor 3, alpha subunit
Calculated molecular weight	72 kDa
Observed molecular weight	72 kDa
GenBank accession number	NM_022462
Gene Symbol	HIF3A
Gene ID (NCBI)	64344
RRID	AB_2880939
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2N7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIF3A, Hypoxia-inducible factor 3-alpha, acts as a transcriptional regulator in adaptive response to low oxygen tension (PMID:11573933, PMID:16126907, PMID:19694616, PMID:20416395, PMID:21069422). It functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. HIF3A also plays a role in the development of the cardiorespiratory system. HIF3A was up-regulated by hypoxia (PMID:16775626). Multiple alternatively spliced transcript variants have been found.

Protocols

Product Specific Protocols
IF protocol for HIF3A antibody 27650-1-AP	Download protocol
IHC protocol for HIF3A antibody 27650-1-AP	Download protocol
WB protocol for HIF3A antibody 27650-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Bioact Mater Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform Authors - Dong Guo View Article
human	WB	Nat Commun Identification of oleoylethanolamide as an endogenous ligand for HIF-3α. Authors - Xiaotong Diao View Article
human	IHC	Theranostics Hypoxia inducible factor (HIF) 3α prevents COPD by inhibiting alveolar epithelial cell ferroptosis via the HIF-3α-GPx4 axis Authors - Junchao Jiang View Article
mouse	IF	Adv Sci (Weinh) An Activity-Dependent NEPAS-PTX3 Axis Links Neurovascular and Myelin Deficits to Cognitive Impairment. Authors - Boya Hu View Article
human	WB	Mol Metab Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort. Authors - Agné Kulyté KD Validated View Article
mouse,human	WB	Mar Life Sci Technol Molecular mechanism of the crosstalk between glucocorticoid receptor (GR) and hypoxia-inducible factor 3α (HIF-3α) pathways. Authors - Meina Zhang View Article

Validation Data Gallery

WB analysis of A431 using 27650-1-AP

WB analysis using 27650-1-AP

WB analysis using 27650-1-AP

IHC staining of mouse kidney using 27650-1-AP

IHC staining of rat cerebellum using 27650-1-AP

IHC staining of rat kidney using 27650-1-AP

IHC staining of rat lung using 27650-1-AP

IHC staining of human lung using 27650-1-AP

IHC staining of human placenta using 27650-1-AP

IHC staining of human placenta using 27650-1-AP

IHC staining of mouse cerebellum using 27650-1-AP

IHC staining of mouse lung using 27650-1-AP

IF Staining of HeLa using 27650-1-AP

HIF3A Polyclonal antibody

HIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform

Identification of oleoylethanolamide as an endogenous ligand for HIF-3α.

Hypoxia inducible factor (HIF) 3α prevents COPD by inhibiting alveolar epithelial cell ferroptosis via the HIF-3α-GPx4 axis

An Activity-Dependent NEPAS-PTX3 Axis Links Neurovascular and Myelin Deficits to Cognitive Impairment.

Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort.

Molecular mechanism of the crosstalk between glucocorticoid receptor (GR) and hypoxia-inducible factor 3α (HIF-3α) pathways.

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