Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis of A431 using 27650-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 27650-1-AP
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 27650-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 27650-1-AP
Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using HIF3A antibody (27650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

HIF3A Polyclonal antibody

HIF3A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 27650-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HIF3 alpha, HIF 3A4, HIF 3A2, HIF 3A, HIF 3 alpha

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Cobalt Chloride treated HeLa cells, A431 cells
Positive IF/ICC detected in	Tunicamycin treated HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 3 publications below

Product Information

27650-1-AP targets HIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26574 Product name: Recombinant human HIF3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 401-486 aa of NM_022462 Sequence: MALAADPRRFCSPDLRRLLGPILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDADALD 相同性解析による交差性が予測される生物種
Full Name	hypoxia inducible factor 3, alpha subunit
Calculated molecular weight	72 kDa
Observed molecular weight	72 kDa
GenBank accession number	NM_022462
Gene Symbol	HIF3A
Gene ID (NCBI)	64344
RRID	AB_2880939
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2N7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIF3A, Hypoxia-inducible factor 3-alpha, acts as a transcriptional regulator in adaptive response to low oxygen tension (PMID:11573933, PMID:16126907, PMID:19694616, PMID:20416395, PMID:21069422). It functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. HIF3A also plays a role in the development of the cardiorespiratory system. HIF3A was up-regulated by hypoxia (PMID:16775626). Multiple alternatively spliced transcript variants have been found.

Protocols

Product Specific Protocols
WB protocol for HIF3A antibody 27650-1-AP	Download protocol
IF protocol for HIF3A antibody 27650-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Identification of oleoylethanolamide as an endogenous ligand for HIF-3α. Authors - Xiaotong Diao View Article
human	WB	Mol Metab Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort. Authors - Agné Kulyté KD Validated View Article
mouse	WB	Front Cell Dev Biol HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring. Authors - Francesca Cuomo KD Validated View Article
mouse	WB	Toxicol Lett Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis. Authors - Wenchao Gu View Article
human	IHC	ERJ Open Res Dynamic analysis of gene signatures in the progression of COPD Authors - Junchao Jiang View Article
mouse	IHC	Bioact Mater Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform Authors - Dong Guo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 27650-1-AP

WB analysis using 27650-1-AP

WB analysis using 27650-1-AP

IF Staining of HeLa using 27650-1-AP

HIF3A Polyclonal antibody

HIF3A Polyclonal Antibody for WB, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of oleoylethanolamide as an endogenous ligand for HIF-3α.

Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort.

HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.

Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis.

Dynamic analysis of gene signatures in the progression of COPD

Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform