Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, MG-63 cells, U2OS cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33973-1-AP targets HICE1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40571 Product name: Recombinant human HICE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-284 aa of BC010176 Sequence: DVGDSEENVQVLDLLSELKDVTAKKDLELRRSFAQVLELSAEASKEAALANQEVWEETQGMAPPSRWYFNQDSACRESGGAPKNTPLSEDDNPGASSAPAQATFISPSEDFSSSSQAEVPPSLSRSGRDLS 相同性解析による交差性が予測される生物種 |
| Full Name | HEC1/NDC80 interacting, centrosome associated 1 |
| Calculated molecular weight | 410 aa, 45 kDa |
| Observed molecular weight | 38kDa, 45kDa |
| GenBank accession number | BC010176 |
| Gene Symbol | HICE1 |
| Gene ID (NCBI) | 93323 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9BT25 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HICE1 antibody 33973-1-AP | Download protocol |
| WB protocol for HICE1 antibody 33973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |