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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIC2 Recombinant antibody (83162-7-RR)

    IF Staining of HepG2 using 83162-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HIC2 Recombinant antibody (83162-7-RR)

    IF Staining of U2OS using 83162-7-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using HIC2 Recombinant antibody (83162-7-RR)

    FC experiment of HepG2 using 83162-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Hic2 Recombinant Antibody (83162-7-RR, Clone:230177E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83162-7-RR

    Affinity and Kinetic Characterization of 83162-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83162-7-RR against Human HIC2 were performed. The affinity constant is 0.527 nM.

HIC2 Recombinant monoclonal antibody

HIC2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83162-7-RR
Clone No.230177E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells, U2OS cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83162-7-RR targets HIC2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18789

Product name: Recombinant human HIC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 161-430 aa of BC094787

Sequence: RSQRLSTASVIQARYQGLVDGRKGAHAPQELPQAKGSDDELFLGGSNQDSVQGLGRAVCPAGGEAGLGGCSSSTNGSSGGCEQELGLDLSKKSPPLPPATPGPHLTPDDAAQLSDSQHGSPPAASAPPVANSASYSELGGTPDEPMDLEGAEDNHLSLLEAPGGQPRKSLRHSTRKKEWGKKEPVAGSPFERREAGPKGPCPGEEGEGVGDRVPNGILASGAGPSGPYGEPPYPCKEEEENGKDASEDSAQSGSEGGSGHASAHYMYRQE

相同性解析による交差性が予測される生物種
Full Name hypermethylated in cancer 2
Calculated molecular weight 615 aa, 66 kDa
GenBank accession numberBC094787
Gene Symbol HIC2
Gene ID (NCBI) 23119
RRIDAB_3670857
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96JB3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumor suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).

Protocols

Product Specific Protocols
IF protocol for HIC2 antibody 83162-7-RRDownload protocol
Standard Protocols
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