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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HGF Polyclonal antibody (26881-1-AP)

    WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26881-1-AP (HGF alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HGF Polyclonal antibody (26881-1-AP)

    WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26881-1-AP (HGF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HGF Polyclonal antibody (26881-1-AP)

    WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26881-1-AP (HGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HGF Polyclonal antibody (26881-1-AP)

    IHC staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26881-1-AP (HGF alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HGF Polyclonal antibody (26881-1-AP)

    IHC staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26881-1-AP (HGF alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HGF Polyclonal antibody (26881-1-AP)

    IHC staining of human tonsillitis using 26881-1-AP (same clone as 26881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26881-1-AP (HGF Alpha antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HGF Polyclonal antibody (26881-1-AP)

    IHC staining of human tonsillitis using 26881-1-AP (same clone as 26881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26881-1-AP (HGF Alpha antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using HGF Polyclonal antibody (26881-1-AP)

    IF Staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using HGF antibody (26881-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using HGF Polyclonal antibody (26881-1-AP)

    IF Staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using HGF antibody (26881-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HGF Polyclonal antibody (26881-1-AP)

    IF Staining of HepG2 using 26881-1-AP (same clone as 26881-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGF Alpha antibody (26881-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

HGF Polyclonal antibody, PBS Only

HGF Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 26881-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

HGF Alpha, Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26881-1-PBS targets HGF in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25456

Product name: Recombinant human HGF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 118-157 aa of BC130286

Sequence: LYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIP

相同性解析による交差性が予測される生物種
Full Name hepatocyte growth factor (hepapoietin A; scatter factor)
Observed molecular weight 69 kDa
GenBank accession numberBC130286
Gene Symbol HGF
Gene ID (NCBI) 3082
RRIDAB_2880668
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP14210
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa α-subunit (containing four kringle domains) and the 34 kDa β-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.

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