Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, MCF-7 cells, MDA-MB-231 cells, human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33841-1-AP targets HFM1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40875 Product name: Recombinant human HFM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 333-518 aa of BC130525 Sequence: LPKYELKVEQITRYSDTTAEILVTVILRNFEQLQTKRTASDSHYVTLIIGDADNQVVYLHKITDSVLLKAGSWAKKIAVKRALKSEDLSINLISSEFVGLDIQQKLTVFYLEPKRFGNQITMQRKSETQISHSKHSDISTIAGPNKGTTASKKPGNRECNHLCKSKHTCGHDCCKIGVAQKSEIKE 相同性解析による交差性が予測される生物種 |
| Full Name | HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) |
| Observed molecular weight | 63-70 kDa |
| GenBank accession number | BC130525 |
| Gene Symbol | HFM1 |
| Gene ID (NCBI) | 164045 |
| RRID | AB_3743089 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | A2PYH4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HFM1 (helicase for meiosis 1), also known as MER3, POF9, Si-11, SEC63D1, and Si-11-6, is a candidate gene of POI. HFM1 was reported to be mainly expressed in germline cells, which is required for crossover formation and complete synapsis of homologous chromosomes in spermiogenesis HFM1, expressed specifically in germline tissues, is a typical ATP-dependent DNA helicase mediating meiosis (PMID: 39725823, 37020390).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HFM1 antibody 33841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |