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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEY2 Recombinant monoclonal antibody (83501-7-RR)

    IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEY2 Recombinant monoclonal antibody (83501-7-RR)

    IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using HEY2 Recombinant monoclonal antibody (83501-7-RR)

    FC experiment of HEK-293 using 83501-7-RR (same clone as 83501-7-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HEY2 Recombinant monoclonal antibody (83501-7-RR)

    FC experiment of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83501-7-RR

    Affinity and Kinetic Characterization of 83501-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83501-7-RR against Human HEY2 were performed. The affinity constant is 1.08 nM.

HEY2 Recombinant monoclonal antibody, PBS Only

HEY2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83501-7-PBS
Clone No.240474F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

240474F2, bHLHb32, Cardiovascular helix-loop-helix factor 1, CHF1, Class B basic helix-loop-helix protein 32

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83501-7-PBS targets HEY2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag30988

Product name: Recombinant human HEY2 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-337 aa of BC007707

Sequence: MKRPCEETTSESDMDETIDVGSENNYSGQSTSSVIRLNSPTTTSQIMARKKRRGIIEKRRRDRINNSLSELRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDAHALAMDFMSIGFRECLTEVARYLSSVEGLDSSDPLRVRLVSHLSTCATQREAAAMTSSMAHHHHPLHPHHWAAAFHHLPAALLQPNGLHASESTPCRLSTTSEVPPAHGSALLTATFAHADSALRMPSTGSVAPCVPPLSTSLLSLSATVHAAAAAATAAAHSFPLSFAGAFPMLPPNAAAAVAAATAISPPLSVSATSSPQQTSSGTNNKPYRPWGTEVGAF

相同性解析による交差性が予測される生物種
Full Name hairy/enhancer-of-split related with YRPW motif 2
Calculated molecular weight 36 kDa
GenBank accession numberBC007707
Gene Symbol HEY2
Gene ID (NCBI) 23493
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBP5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system.

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