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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEY2 Recombinant antibody (83501-7-RR)

    IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEY2 Recombinant antibody (83501-7-RR)

    IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using HEY2 Recombinant antibody (83501-7-RR)

    FC experiment of HEK-293 using 83501-7-RR (same clone as 83501-7-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HEY2 Recombinant antibody (83501-7-RR)

    FC experiment of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83501-7-RR

    Affinity and Kinetic Characterization of 83501-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83501-7-RR against Human HEY2 were performed. The affinity constant is 1.08 nM.

HEY2 Recombinant monoclonal antibody, PBS Only

HEY2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83501-7-PBS
Clone No.240474F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1, bHLHb32

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83501-7-PBS targets HEY2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag30988

Product name: Recombinant human HEY2 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-337 aa of BC007707

Sequence: MKRPCEETTSESDMDETIDVGSENNYSGQSTSSVIRLNSPTTTSQIMARKKRRGIIEKRRRDRINNSLSELRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDAHALAMDFMSIGFRECLTEVARYLSSVEGLDSSDPLRVRLVSHLSTCATQREAAAMTSSMAHHHHPLHPHHWAAAFHHLPAALLQPNGLHASESTPCRLSTTSEVPPAHGSALLTATFAHADSALRMPSTGSVAPCVPPLSTSLLSLSATVHAAAAAATAAAHSFPLSFAGAFPMLPPNAAAAVAAATAISPPLSVSATSSPQQTSSGTNNKPYRPWGTEVGAF

相同性解析による交差性が予測される生物種
Full Name hairy/enhancer-of-split related with YRPW motif 2
Calculated molecular weight 36 kDa
GenBank accession numberBC007707
Gene Symbol HEY2
Gene ID (NCBI) 23493
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBP5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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