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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEXB Polyclonal antibody (16229-1-AP)

    WB analysis using 16229-1-AP (same clone as 16229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HEXB Polyclonal antibody (16229-1-AP)

    WB analysis of HeLa using 16229-1-AP (same clone as 16229-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using HEXB Polyclonal antibody (16229-1-AP)

    WB analysis of mouse lung using 16229-1-AP (same clone as 16229-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HEXB Polyclonal antibody (16229-1-AP)

    WB analysis of HEK-293 using 16229-1-AP (same clone as 16229-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using HEXB Polyclonal antibody (16229-1-AP)

    WB analysis of mouse kidney using 16229-1-AP (same clone as 16229-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HEXB Polyclonal antibody (16229-1-AP)

    WB analysis of Jurkat using 16229-1-AP (same clone as 16229-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HEXB Polyclonal antibody (16229-1-AP)

    IHC staining of human kidney using 16229-1-AP (same clone as 16229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16229-1-AP (HEXB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HEXB Polyclonal antibody (16229-1-AP)

    IHC staining of human kidney using 16229-1-AP (same clone as 16229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16229-1-AP (HEXB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HEXB Polyclonal antibody, PBS Only

HEXB Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16229-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16229-1-PBS targets HEXB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8683

Product name: Recombinant human HEXB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-332 aa of BC017378

Sequence: MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSF

相同性解析による交差性が予測される生物種
Full Name hexosaminidase B (beta polypeptide)
Calculated molecular weight 556 aa, 63 kDa
Observed molecular weight 63-67 kDa
GenBank accession numberBC017378
Gene Symbol HEXB
Gene ID (NCBI) 3074
RRIDAB_2264075
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07686
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The HEXB (Beta-hexosaminidase subunit beta) gene encodes the beta subunit of the enzyme hexosaminidase, which is involved in the breakdown of gangliosides. It is responsible for the degradation of GM2 gangliosides, and has a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Refer to the domains of HEXB and glycosylation, there will be some different bands with MW 63-67 kDa precursor; 59-63 kDa signal peptide removed; 50-55 kDa subunit beta (or signal and propeptide removed); 20-25 kDa subunit beta chain B; ~30 kDa subunit beta chain A (Uniprot).

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