Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18101-1-AP targets HERV-FRD in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12380 Product name: Recombinant human HERV-FRD protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 14-353 aa of BC068585 Sequence: LAAYRHPDFPLLEKAQQLLQSTGSPYSTNCWLCTSSSTETPGTAYPASPREWTSIEAELHISYRWDPNLKGLMRPANSLLSTVKQDFPDIRQKPPIFGPIFTNINLMGIAPICVMAKRKNGTNVGTLPSTVCNVTFTVDSNQQTYQTYTHNQFRHQPRFPKPPNITFPQGTLLDKSSRFCQGRPSSCSTRNFWFRPADYNQCLQISNLSSTAEWVLLDQTRNSLFWENKTKGANQSQTPCVQVLAGMTIATSYLGISAVSEFFGTSLTPLFHFHISTCLKTQGAFYICGQSIHQCLPSNWTGTCTIGYVTPDIFIAPGNLSLPIPIYGNSPLPRVRRAIH 相同性解析による交差性が予測される生物種 |
| Full Name | HERV-FRD provirus ancestral Env polyprotein |
| Calculated molecular weight | 538 aa, 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC068585 |
| Gene Symbol | HERV-FRD |
| Gene ID (NCBI) | 405754 |
| RRID | AB_3741946 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60508 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HERV-FRD (Human Endogenous Retrovirus FRD Envelope Protein) belongs to the W family of the HERV family. Also known as Syncytin-2, this protein is a key member of the syncytin family and is encoded by an env gene from an ancient retrovirus integrated into the host genome. It primarily mediates cell-cell fusion, which is essential for forming the placental syncytiotrophoblast and maintaining normal pregnancy. Furthermore, HERV-FRD exhibits immunosuppressive functions: its transmembrane subunit contains an immunosuppressive domain that inhibits maternal immune rejection of the fetus and protects the placenta from immune attack. Abnormal expression of HERV-FRD is associated with pregnancy-related disorders such as preeclampsia and intrauterine growth restriction, as well as with the development and progression of certain cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HERV-FRD antibody 18101-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |