HERC5 Polyclonal antibody

HERC5 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22692-1-AP

Synonyms

CEB1, CEBP1, Cyclin E binding protein 1, E3 ISG15 protein ligase HERC5, hect domain and RLD 5, HERC5



Tested Applications

Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22692-1-AP is HERC5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HERC5 fusion protein Ag18449 相同性解析による交差性が予測される生物種
Full Name hect domain and RLD 5
Calculated molecular weight 1024 aa, 117 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC140716
Gene symbol HERC5
Gene ID (NCBI) 51191
RRIDAB_2879151
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for HERC5 antibody 22692-1-APDownload protocol
IP protocol for HERC5 antibody 22692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

MBio

ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.

Authors - Edmund Osei Kuffour
humanWB

Int J Mol Med

ISG15 inhibits cancer cell growth and promotes apoptosis.

Authors - Mei-Juan Zhou
human,mouseWB,CoIP

J Clin Invest

Posttranslational ISGylation of NLRP3 by HERCs enzymes facilitates inflammasome activation in models of inflammation

Authors - Ying Qin
  • KD Validated
humanWB

Cell Rep

Regulation of STING activity in DNA sensing by ISG15 modification

Authors - Chaohui Lin