Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
27459-1-AP targets HERC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26501 Product name: Recombinant human LOC440248 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 169-335 aa of BC047911 Sequence: AGSKLEKHKIKKSTKQAFAGQVDLDLLRCQQLKLYILKAGRALFSHQDKLRQILSQPAVQETGSVHTDDGAVVSPDLGDMSPEGPQPPMILLQQLLASATQPSPVKAIFDKQELEAAALAVCQCLAVESTHPSSPGFEDCSSSEATTPVAVQHIRPARVKRRKQSPV 相同性解析による交差性が予測される生物種 |
| Full Name | hect domain and RLD 2 |
| Calculated molecular weight | 527 kDa |
| Observed molecular weight | 527 kDa |
| GenBank accession number | BC047911 |
| Gene Symbol | HERC2 |
| Gene ID (NCBI) | 8924 |
| RRID | AB_2918123 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95714 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HERC2 antibody 27459-1-AP | Download protocol |
| WB protocol for HERC2 antibody 27459-1-AP | Download protocol |
| IF protocol for HERC2 antibody 27459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma | ||
Adv Sci (Weinh) Loss of MNX1 Sensitizes Tumors to Cytotoxic T Cells by Degradation of PD-L1 mRNA | ||
Free Radic Biol Med Cryptochrome 1 regulates ovarian granulosa cell senescence through NCOA4-mediated ferritinophagy |