Validation Data Gallery
Tested Applications
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
11148-1-AP targets Hephaestin in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1604 Product name: Recombinant human HEPH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 859-1158 aa of BC011561 Sequence: TYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ 相同性解析による交差性が予測される生物種 |
| Full Name | hephaestin |
| Calculated molecular weight | 130 kDa |
| GenBank accession number | BC011561 |
| Gene Symbol | Hephaestin |
| Gene ID (NCBI) | 9843 |
| RRID | AB_11064728 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQS7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Hephaestin, also named as KIAA0698, belongs to the multicopper oxidase family. It is provied as ferroxidase of cellular iron export. HEPH is implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. HEPE is active in placenta but may not play a key role in placental iron transport.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Hephaestin antibody 11148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Biochem Funct Ferroportin 1 and hephaestin expression in BeWo cell line with different iron treatment. | ||
Chin Herb Med Neuroprotective effects of kukoamine A on 6-OHDA-induced Parkinson's model through apoptosis and iron accumulation inhibition |