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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HELZ Polyclonal antibody (26635-1-AP)

    WB analysis of HeLa using 26635-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26635-1-AP (HELZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HELZ Polyclonal antibody (26635-1-AP)

    WB analysis of HeLa using 26635-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26635-1-AP (HELZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HELZ Polyclonal antibody (26635-1-AP)

    IHC staining of human breast cancer using 26635-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26635-1-AP (HELZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HELZ Polyclonal antibody (26635-1-AP)

    IHC staining of human breast cancer using 26635-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26635-1-AP (HELZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HELZ Polyclonal antibody (26635-1-AP)

    IF Staining of HepG2 using 26635-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HELZ antibody (26635-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HELZ Polyclonal antibody

HELZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26635-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0054, HUMORF5, DRHC, DHRC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26635-1-AP targets HELZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24326

Product name: Recombinant human HELZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC130582

Sequence: MEDRRAEKSCEQACESLKRQDYEMALKHCTEALLSLGQYSMADFTGPCPLEIERIKIESLLYRIASFLQLKNYMQADEDCRHVLGEGLAKGEDAFRAVLCCM

相同性解析による交差性が予測される生物種
Full Name helicase with zinc finger
Calculated molecular weight 1942 aa, 219 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC130582
Gene Symbol HELZ
Gene ID (NCBI) 9931
RRIDAB_2880584
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP42694
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HELZ antibody 26635-1-APDownload protocol
IHC protocol for HELZ antibody 26635-1-APDownload protocol
IF protocol for HELZ antibody 26635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols