Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of K-562 using 11955-1-AP (same clone as 11955-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
WB analysis of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 11955-1-AP (same clone as 11955-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
WB analysis of DU 145 using 11955-1-AP (same clone as 11955-1-PBS)
DU 145 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IP experiment of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)
IP result of anti-HELLS (IP:11955-1-AP, 4ug; Detection:11955-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IHC staining of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IHC staining of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 11955-1-AP (same clone as 11955-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 11955-1-AP (same clone as 11955-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IHC staining of rat thymus using 11955-1-AP (same clone as 11955-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IHC staining of rat thymus using 11955-1-AP (same clone as 11955-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 11955-1-AP (same clone as 11955-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HELLS antibody (11955-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 11955-1-AP (same clone as 11955-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HELLS Polyclonal antibody (11955-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.
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1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HELLS Polyclonal antibody (11955-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

HELLS Polyclonal antibody, PBS Only

HELLS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 11955-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11955-1-PBS targets HELLS in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2558 Product name: Recombinant human HELLS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC031004 Sequence: MFGSSEKETIELSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPYLIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSRLDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF 相同性解析による交差性が予測される生物種
Full Name	helicase, lymphoid-specific
Calculated molecular weight	348 aa, 40 kDa, 97 kDa
Observed molecular weight	97 kDa
GenBank accession number	BC031004
Gene Symbol	HELLS
Gene ID (NCBI)	3070
RRID	AB_2117529
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NRZ9
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 11955-1-AP (same clone as 11955-1-PBS)

WB analysis of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)

WB analysis of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

WB analysis of PC-3 using 11955-1-AP (same clone as 11955-1-PBS)

WB analysis of DU 145 using 11955-1-AP (same clone as 11955-1-PBS)

IP experiment of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

IHC staining of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)

IHC staining of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)

IHC staining of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

IHC staining of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

IHC staining of rat testis using 11955-1-AP (same clone as 11955-1-PBS)

IHC staining of rat testis using 11955-1-AP (same clone as 11955-1-PBS)

IHC staining of rat thymus using 11955-1-AP (same clone as 11955-1-PBS)

IHC staining of rat thymus using 11955-1-AP (same clone as 11955-1-PBS)

IF Staining of HepG2 using 11955-1-AP (same clone as 11955-1-PBS)

FC experiment of HepG2 using 11955-1-AP (same clone as 11955-1-PBS)