Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, MOLT-4 cells, EL-4 cells |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87876-1-RR targets HELIOS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4087 Product name: Recombinant human IKZF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 227-526 aa of BC028936 Sequence: NNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH 相同性解析による交差性が予測される生物種 |
| Full Name | IKAROS family zinc finger 2 (Helios) |
| Calculated molecular weight | 500 aa, 57 kDa |
| Observed molecular weight | 65-75 kDa |
| GenBank accession number | BC028936 |
| Gene Symbol | HELIOS |
| Gene ID (NCBI) | 22807 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UKS7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HELIOS, encoded by the IKZF2 gene (Ikaros Family Zinc Finger Protein 2), is a transcription factor belonging to the Ikaros family of zinc-finger proteins. It is primarily expressed in hematopoietic cells, particularly in regulatory T cells (Tregs) and early lymphoid progenitors, where it plays a critical role in immune regulation, T-cell development, and the maintenance of Treg suppressive function. HELIOS binds to specific DNA sequences to regulate gene expression involved in lymphocyte differentiation and immune homeostasis. It is also widely used as a stable marker for activated Tregs. Dysregulation of HELIOS has been implicated in autoimmune diseases, allergy, and cancer immunotherapy resistance.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HELIOS antibody 87876-1-RR | Download protocol |
| WB protocol for HELIOS antibody 87876-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |