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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HELB Polyclonal antibody

HELB Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 24700-1-AP

Synonyms

DNA helicase B, hDHB, HELB, helicase (DNA) B

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 24700-1-AP

    WB analysis of Jurkat using 24700-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24700-1-AP

    WB analysis of HeLa using 24700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 24700-1-AP

    WB analysis of HEK-293 using 24700-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 24700-1-AP

    IP experiment of HEK-293 using 24700-1-AP

    IP result of anti-HELB (IP:24700-1-AP, 4ug; Detection:24700-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

  • IF Staining of HeLa using 24700-1-AP

    IF Staining of HeLa using 24700-1-AP

    Immunofluorescent analysis of HeLa cells using 24700-1-AP (HELB antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24700-1-AP is HELB Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HELB fusion protein Ag20335 相同性解析による交差性が予測される生物種
Full Name helicase (DNA) B
Calculated molecular weight 1087 aa, 123 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberBC157019
Gene symbol HELB
Gene ID (NCBI) 92797
RRIDAB_2879680
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HELB antibody 24700-1-APDownload protocol
IF protocol for HELB antibody 24700-1-APDownload protocol
IP protocol for HELB antibody 24700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols