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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HELB Polyclonal antibody (24700-1-AP)

    WB analysis of Jurkat using 24700-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HELB Polyclonal antibody (24700-1-AP)

    WB analysis of HeLa using 24700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HELB Polyclonal antibody (24700-1-AP)

    WB analysis of HEK-293 using 24700-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HELB Polyclonal antibody (24700-1-AP)

    IP experiment of HEK-293 using 24700-1-AP

    IP result of anti-HELB (IP:24700-1-AP, 4ug; Detection:24700-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HELB Polyclonal antibody (24700-1-AP)

    IF Staining of HeLa using 24700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HELB antibody (24700-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

HELB Polyclonal antibody

HELB Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 24700-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

hDHB, EC:3.6.4.12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24700-1-AP targets HELB in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20335

Product name: Recombinant human HELB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC157019

Sequence: MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDAEELCSGGVKAGSLPGCLRVSICDENTQETCKVFGRFPITGAWWRVKVQVKPVVGSRSYQYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDDVNKFLTWVKEVSNYKNLNFENLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMTALQFPKIMEFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIAFCQCESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASESLKFLKDIGVVTYEKSC

相同性解析による交差性が予測される生物種
Full Name helicase (DNA) B
Calculated molecular weight 1087 aa, 123 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberBC157019
Gene Symbol HELB
Gene ID (NCBI) 92797
RRIDAB_2879680
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG08
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HELB antibody 24700-1-APDownload protocol
IF protocol for HELB antibody 24700-1-APDownload protocol
IP protocol for HELB antibody 24700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols