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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using HECW1 Polyclonal antibody (24695-1-AP)

    IHC staining of human urothelial carcinoma using 24695-1-AP (same clone as 24695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using HECW1 Polyclonal antibody (24695-1-AP)

    IHC staining of human urothelial carcinoma using 24695-1-AP (same clone as 24695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HECW1 Polyclonal antibody (24695-1-AP)

    IHC staining of mouse cerebellum using 24695-1-AP (same clone as 24695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HECW1 Polyclonal antibody (24695-1-AP)

    IHC staining of mouse cerebellum using 24695-1-AP (same clone as 24695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24695-1-PBS in a different storage buffer formulation.

HECW1 Polyclonal antibody, PBS Only

HECW1 Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 24695-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24695-1-PBS targets HECW1 in IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20264

Product name: Recombinant human HECW1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC151227

Sequence: MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDD

相同性解析による交差性が予測される生物種
Full Name HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Calculated molecular weight 1606 aa, 180 kDa
GenBank accession numberBC151227
Gene Symbol HECW1
Gene ID (NCBI) 23072
RRIDAB_2918085
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ76N89
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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