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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR)

    WB analysis using 83859-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83859-1-RR (HEC1/HEC/NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR)

    IF Staining of HepG2 using 83859-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR)

    IF Staining of HepG2 using 83859-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR)

    IF Staining of HepG2 using 83859-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR)

    IF Staining of HeLa using 83859-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Flow cytometry (FC) experiment of HeLa cells using HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR)

    FC experiment of HeLa using 83859-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR, Clone:5A2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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HEC1/HEC/NDC80 Recombinant antibody

HEC1/HEC/NDC80 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83859-1-RR
Clone No.5A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

NDC80, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, Jurkat cells, NCCIT cells, K-562 cells, HEK-293T cells, A431 cells, MCF-7 cells, HepG2 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83859-1-RR targets HEC1/HEC/NDC80 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name NDC80 homolog, kinetochore complex component (S. cerevisiae)
Observed molecular weight 74 kDa
GenBank accession number/
Gene Symbol NDC80
Gene ID (NCBI) 10403
RRIDAB_3671443
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDC80 complex is a heterotetrameric protein complex comprised of Hec1 (also called Ndc80), Nuf2, Spc24, and Spc25. Mis12 complex (bound to CENP-C or CENP-T) or CDK1-phosphorylated CENP-T to anchor the NDC80 complex to the kinetochore. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

Protocols

Product Specific Protocols
WB protocol for HEC1/HEC/NDC80 antibody 83859-1-RRDownload protocol
IF protocol for HEC1/HEC/NDC80 antibody 83859-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols