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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEATR6 Polyclonal antibody (22560-1-AP)

    WB analysis using 22560-1-AP (same clone as 22560-1-PBS)

    MCF-7 cells lysate was subjected to SDS PAGE followed by western blot with 22560-1-AP (HEATR6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22560-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using HEATR6 Polyclonal antibody (22560-1-AP)

    IP experiment of MCF-7 using 22560-1-AP (same clone as 22560-1-PBS)

    IP result of anti-HEATR6 (IP:22560-1-AP, 4 ug; Detection:22560-1-AP 1:1000) with MCF-7 cells lysate 1520 ug. This data was developed using the same antibody clone with 22560-1-PBS in a different storage buffer formulation.

HEATR6 Polyclonal antibody, PBS Only

HEATR6 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 22560-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

ABC1, HEAT repeat containing 6, HEAT repeat-containing protein 6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22560-1-PBS targets HEATR6 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18437

Product name: Recombinant human HEATR6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 832-1181 aa of BC119813

Sequence: AATSRALGVYVLFPCLRQDVIFVADAANAILMSLEDKSLNVRAKAAWSLGNLTDTLIVNMETPDPSFQEEFSGLLLLKMLRSAIEASKDKDKVKSNAVRALGNLLHFLQPSHIEKPTFAEIIEESIQALISTVLTEAAMKVRWNACYAMGNVFKNPALPLGTAPWTSQAYNALTSVVTSCKNFKVRIRSAAALSVPGKREQYGSVDQYARIWNALVTALQKSEDTIDFLEFKYCVSLRTQICQALIHLLSLASASDLPCMKETLELSGNMVQSYILQFLKSGAEGDDTGAPHSPQERDQMVRMALKHMGSIQAPTGDTARRAIMGFLEEILAVCFDSSGSQGALPGLTNQ

相同性解析による交差性が予測される生物種
Full Name HEAT repeat containing 6
Calculated molecular weight 1181 aa, 129 kDa
Observed molecular weight129 kDa
GenBank accession numberBC119813
Gene Symbol HEATR6
Gene ID (NCBI) 63897
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ6AI08
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HEAT repeat-containing protein 6 (HEATR6) is amplified in certain breast cancer cell lines, such as MCF-7 and BT-474, suggesting its role in cancer progression. Additionally, HEATR6 is implicated in the regulation of gene expression, particularly in the context of long non-coding RNAs (lncRNAs) associated with breast cancer (PMID: 30953646).

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