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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEATR5B Polyclonal antibody (32316-1-AP)

    WB analysis using 32316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32316-1-AP (HEATR5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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HEATR5B Polyclonal antibody

HEATR5B Polyclonal Antibody for WB, ELISA
Cat No. 32316-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HEAT repeat-containing protein 5B, KIAA1414, p200, p200a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, U-251 cells, RT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32316-1-AP targets HEATR5B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEATR5B fusion protein Ag36352 相同性解析による交差性が予測される生物種
Full Name HEAT repeat containing 5B
Observed molecular weight~210 kDa
GenBank accession numberBC150605
Gene Symbol HEATR5B
Gene ID (NCBI) 54497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9P2D3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HEATR5B (HEAT Repeat Containing 5B) is a protein containing HEAT repeat that binds directly to the dynein tail and dynactin and has an evolutionarily conserved function in promoting the motility of adaptor protein-1 (AP1)-associated endosomal membranes (PMID: 37872872).

Protocols

Product Specific Protocols
WB protocol for HEATR5B antibody 32316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols