Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, U-251 cells, RT-4 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32316-1-AP targets HEATR5B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36352 Product name: Recombinant human HEATR5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 700-850 aa of BC150605 Sequence: FTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVR 相同性解析による交差性が予測される生物種 |
| Full Name | HEAT repeat containing 5B |
| Observed molecular weight | ~210 kDa |
| GenBank accession number | BC150605 |
| Gene Symbol | HEATR5B |
| Gene ID (NCBI) | 54497 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9P2D3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HEATR5B (HEAT Repeat Containing 5B) is a protein containing HEAT repeat that binds directly to the dynein tail and dynactin and has an evolutionarily conserved function in promoting the motility of adaptor protein-1 (AP1)-associated endosomal membranes (PMID: 37872872).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HEATR5B antibody 32316-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |