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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using HDHD2 Polyclonal antibody (26147-1-AP)

    WB analysis of mouse liver using 26147-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26147-1-AP (HDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HDHD2 Polyclonal antibody (26147-1-AP)

    WB analysis of HepG2 using 26147-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26147-1-AP (HDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDHD2 Polyclonal antibody (26147-1-AP)

    IF Staining of HeLa using 26147-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDHD2 antibody (26147-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HDHD2 Polyclonal antibody

HDHD2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26147-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Haloacid dehalogenase-like hydrolase domain-containing protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26147-1-AP targets HDHD2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23518

Product name: Recombinant human HDHD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-169 aa of BC033031

Sequence: MLLVDDRALPDFKGIQTSDPNAVVMGLAPEHFHYQILNQAFRLLLDGAPLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKPEKTFFLEALRGTGCEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRASDEEKINPPPYLTCESFPHAVDHILQHLL

相同性解析による交差性が予測される生物種
Full Name haloacid dehalogenase-like hydrolase domain containing 2
Observed molecular weight 18 kDa, 28 kDa
GenBank accession numberBC033031
Gene Symbol HDHD2
Gene ID (NCBI) 84064
RRIDAB_2880404
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0R4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HDHD2 antibody 26147-1-APDownload protocol
IF protocol for HDHD2 antibody 26147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols