Validation Data Gallery
Tested Applications
| Positive WB detected in | Ramos cells, A549 cells, Caco-2 cells, Calu-1 cells, Raji cells |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33953-1-AP targets HDHD1A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40679 Product name: Recombinant human HDHD1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC012494 Sequence: MDGLLLDTERLYSVVFQEICNRYDKKYSWDVKSLVMGKKALEAAQIIIDVLQLPMSKEELVEESQTKLKEVFPTAALMPGAEKLIIHLRKHGIPFALATSSRSASFDMKTSRHKEFFSLFSHIVLGDDPEVQHGKPDPDIFLACAKRFSPPPAMEKCLVFEDAPNGVEAALAAGMQVVMVPDGNLSRDLTTKATLVLNSLQDFQPELFGLPSYE 相同性解析による交差性が予測される生物種 |
| Full Name | haloacid dehalogenase-like hydrolase domain containing 1A |
| Calculated molecular weight | 228 aa, 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC012494 |
| Gene Symbol | HDHD1A |
| Gene ID (NCBI) | 8226 |
| RRID | AB_3743122 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q08623 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The enzyme encoded by human HDHD1A (hHDHD1A; also known as HDHD1, PUDP, GS1; Gene ID: 8226) has activity specific for ΨMP 5'-phosphatase (PMID: 20722631). HDHD1A belongs to the HAD-like hydrolase superfamily. In humans, the enzyme HDHD1A dephosphorylates pseudouridine 5'-monophosphate (ΨMP), and the resulting Ψ is excreted in the urine (PMID: 40409548).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HDHD1A antibody 33953-1-AP | Download protocol |
| WB protocol for HDHD1A antibody 33953-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |